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Objective To establish an artificial intelligence deep learning model using clinical colonoscopy images and video to assist the diagnosis by colonoscopy. Methods More than 600000 colonoscopy images were collected in endoscopic center of the Second Affiliated Hospital of Zhejiang University School of Medicine from 2014 to 2018, and endoscopic experts recorded a large number of high-quality operation video of colonoscopy as analysis data. After repeated discussion by six experts, the classified intestinal sites and pathological features were determined, and fuzzy and confusable images were deleted. The final selection result was approximately 1 out of 4. And then the features of images were marked using an independently developed software. The deep learning algorithm was developed using TensorFlow platform of Google. Results After repeated comparison and analysis of the results of machine training and judgment results combined with pathology from endoscopic experts, the sensitivity of the model for some diseases ( such as colon polyps) was 99％ under laboratory conditions. In the clinical colonoscopy test, the sensitivity, specificity, and overall accuracy of this model for diagnosis of colon polyps were 98. 30％(4187/4259), 88. 10％ (17620/20000), and 92. 92％ [2×98. 30％×88. 10％/(98. 30％+88. 10％)], respectively. The sensitivity and specificity for ulcerative colitis were 78. 32％ ( 2671/3410) , and 67. 06％(13412/20000), respectively. The diagnosis time spent on a single image was 0. 5±0. 03 s, and it was the real time for application, including system recognition, text prompt in video image, background record and storage. Conclusion The artificial intelligence assisted diagnosis model developed by our team can identify colonic polyps, colorectal cancer, colorectal eminence, colonic diverticulum, ulcerative colitis, etc. The auxiliary diagnosis model of colon disease can guide beginners to carry out colonoscopy, and can improve lesion detection rate, reduce misdiagnosis rate, and improve the overall operating efficiency of endoscopic center, which is conducive to the quality control of colonoscopy.
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Objective To investigate radiation-induced carotid and cerebral vascular injury and its relationship with radiation-induced temporal lobe necrosis in nasopharyngeal carcinoma (NPC) patients.Methods Fifty-eight NPC patients with radiation-induced temporal lobe necrosis (TLN) were recruited in the study.Duplex ultrasonography was used to scan bilateral carotid arterials to evaluate the intima-media thickness (IMT) and occurrence of plaque formation.Flow velocities of bilateral middle cerebral arteries (MCAs),internal carotid arteries (ICAs) and basal artery (BA) were estimated through Transcranial Color Doppler (TCD).The result were compared with data from 33 patients who were free from radiation-induced temporal lobe necrosis after radiotherapy and 29 healthy individuals,to study the relationship between IMT,occurrence of plaque and ventricular diastolic dysfunction.Results Significant differences in IMT,occurrence of plaques of ICAs and flow velocities of both MCAs and ICAs were found between patients after and healthy individuals (t =18.624,8.221,P ＜0.05;x2 =17.886,P ＜0.05;t =14.367,10.112,P ＜0.05).IMT had positive correlation with post radiation interval (r =0.368,P =0.049).IMT or incidence of plaques may be related to dose of radiotherapy,course of treatment and T stage (x2 =-17.635,12.006,-3.125,P ＜ 0.05).Compared with result from patients without radiation-induced TLN,the mean IMT was significantly thicker in patients with TLN (t =10.208,P ＜ 0.05).Plaques were more common in patients with TLN than patients without TLN (x2 =13.118,P ＜0.05).In addition,flow velocities of MCAs and ICAs in patients with TLN were much faster (t =5.011,5.035,P ＜ 0.05).Among patients with unilateral TLN,flow velocity of MCAs was significantly different between ipsilateral and contralateral sides to the lesion (t =18.362,P ＜ 0.05).Conclusions Thickening of IMT,occurrence of plaque formation and hemodynamic abnormality are more common in patients after radiotherapy,especially in those with TLN,compared with healthy individuals.
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Objective To explore the effect of TGF-β microbubble targeting on the treatment of skeletal muscle injury in rats.MethodsEighty SD rats of 2 months old were randomly divided into experimental group(including TGF-β microbubble),pure drug group,microbubble group(exclusive drug) and control group,20 SD rats in each group.Four groups accepted ultrasonic transmission with consistent time and frequency.The contraction stress and stress relaxation of injured gastrocnemius muscle were measured,the changes of injured gastrocnemius muscle were observed by light microscope and electron microscope.Results There was no statistically significant difference in contraction stress of gastrocnemius muscle among all groups at 1,5,9 days after injury(P>0.05).And at 14 days,the contraction stress of gastrocnemius muscle of the experimental group and the pure drug group were obviously higher than those of the rest two groups,the differences were significant(P<0.05).The difference of gastrocnemius muscle contraction stress was statistically significant between the experimental group and the pure drug group(P<0.05).For the stress relaxation of gastrocnemius muscle,the experimental group was lower than the other three groups at 1 day after injury,the differences were significant(P<0.05).Light microscopy showed the number of muscle fiber in the experimental group increased obviously at 9 days after injury.There was no scarring at 14 days after injury.Electron microscope showed mitochondria and sarcoplasmic reticulum increased in the experimental group,and the new muscle cells and satellite cells were significantly more than those of the rest three groups.Conclusion TGF-β cell targeted therapy can obviously improve the shrinkage stress of skeletal muscle in rats after injury and recover the stress decay,which can obviously promote the injury repair and regeneration of skeletal muscle,and reduce scar formation at the same time.
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@#Recent advances in nanotechnology have greatly facilitated the development of nanoparticle drug delivery system. The present work reviews recent literature on nanotechnology-based applications and developments of silymarin preparation. A variety of nanoparticle formulations have been extensively investigated to improve the absorption and bioavalability of silymarin, including: improving the drug dissolution signficantly(such as nanosuspensions, inclusion complex with β-cyclodextrins, self-emulsifying drug delivery systems, solid dispersions, micronization), promoting the permeability across cell membrane(such as complexation with phospholipids, nanostructured lipid carriers)and other methods(such as micelles, mesoporous silica nanoparticles, polyamidoamine dendrimers). The applications of nanoscience and nanotechnology can help to promote the further pharmaceutical development of silymarin.
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The relationship between subclinical hypothyroidism (SCH) and the components of metabolic syndrome (MS) was explored.A total of 2 252 subjects aged 20 to 79 years from medical examination were identified.The prevalence of SCH was 4.88％.The rate of 7.30％ in female was markedly higher than 2.17％ in male(P＜0.01).The highest SCH prevalence in adults was in the ≥70 years old group.The prevalence of SCH in female was increased with age(P＜0.01).Compared with the euthyroid group,body mass index,triglyceride,and low density lipoprotein-cholesterol were significantly elevated (P＜0.05),while high density lipoprotein-cholesterol was significantly decreased in SCH group(P＜0.05).The incidences of hypertension,dyslipidosis,and MS in SCH group were significantly higher than those in euthyroid group(P＜0.05 or P＜0.01) ; Serum TSH in the MS group was higher than that in the Non-MS group[(2.43 ± 1.55 vs 2.21 ± 1.48) mU/L,P＜0.01].The incidence of SCH in MS group was significantly higher than that in the Non-MS group (7.69％ vs 4.25％,P＜0.05).The prevalence of SCH in women was increased with age.Multiple factors of dysbolism were clustered in subjects with SCH.There is a correlation between SCH and MS.
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Objective:To determine the effect of Chuju total flavonoids (CJTF) on the secreted frizzled-related protein 4 (SFRP4) expression in Wnt pathway in rheumatoid arthritis (AR) model rats. Methods:hTe role of CJTF in the treatment of AR model rats was evaluated by rat arthritis score and paw edema score. The expression regulation of the SFRP4,β-catenin and C-myc in Wnt pathway in AR model rats was detected by RT-PCR and Western blot atfer CJTF gavage treatment. Results:Atfer CJTF treatment, the rat arthritis score and paw edema score in AR model rats were signiifcantly decreased when the AR model rats were treated with CJTF, the SFRP4 expression was signiifcantly up-regulated, while theβ-catenin and C-myc gene expression were signiifcantly down-regulated in AR model rat synovial tissues. Conclusion:CJTF has significant therapeutic effect and inhibitory effect on Wnt pathway activation by targeting SFRP4 in AR model rat synovium.
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ObjectiveTo evaluate the clinical features of hyperuricemia (HUA) and relationship between serum uric acid (SUA) and metabolic syndrome (MS) in adults.MethodsData were gathered from medical examination among adults age ≥ 20 years,and totally 18 862 subjects were analyzed in this study.ResultsThe prevalence of HUA was 14.8％ (2788/18 862).The rate was 18.4％( 1926/10 445) in men,10.2％ (862/8417) in women.The prevalence of HUA was increased with age,there was significant difference among different age (P =0.000),the rate in men was higher than that in women (P ＜ 0.01 or ＜0.05 ).Compared with normal SUA Patients,the levels of body mass index(BMI),systolic blood pressure (SBP),diastolic blood pressure (DBP),total cholesterol (TC),triacylglycerol (TG) and lowdensity lipid cholesterol(LDL-C) were markedly elevated (P ＜0.01 or ＜0.05),and the level of highdensity lipid cholesterol (HDL-C) was markedly decreased in HUA patients (P ＜0.05).Stepwise regression analysis showed that BMI,SBP,DBP,fasting plasma glucose (FPG),TG,HDL-C were the independent correlation factor of SUA.The incidence of hypertension,over weight/obesity,dyslipidosis,hyperglycosemia and MS in HUA patients [24.1％ (672/2788),36.2％ (1009/2788),31.2％ (870/2788),18.0％ (502/2788),20.8％(580/2788) ] was significantly higher than that in normal SUA patients [ 18.2％ (2925/16 074),26.4％(4244/16 074),22.0％(3536/16 074),15.00(2411/16 074),17.0％(2733/16 074),P＜ 0.01 ].The level of SUA in patients with MS [ (342.3 ± 64.2 ) μ mol/L ] was higher than that of those without MS[ (319.3 ± 67.1 )μ mol/L,P =0.000].ConclusionsThe prevalence of HUA is increased with age.Multiple factors of dysbolism are clustered in subjects with HUA.There is correlation between SUA and MS.
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Objective To investigate the association between obesity and benign prostate hyperplasia (BPH).Methods A total of 253 men (>40 year old) with no obesity related diseases were selected.Medical history,height,body weight,waist circumference (WC),body fat ratio,blood pressure,fasting blood glucose,blood lipid and prostate ultrasound were evaluated.The participants were then assigned to the normal group (body mass index 18.5 to 23.9 kg/m2),overweight group (24.0 to 27.9 kg/m2) and obesity group (≥28 kg/m2).The subjects were also divided into the normal group (WC25%).Prostate volume was analyzed in each group.Results The prostate volume was significantly different between the normal body mass index group and overweight or obesity groups,normal WC group and abdominal obesity group,and normal body fat ratio group and excessive body fat ratio group.The prostate volume was increased in individuals with higher body mass index,WC and body fat ratio.Of those with body mass index≥24 kg/m2 and normal WC or body fat ratio,the incidence of BPH was not significantly increased.Conclusion Obesity may be a risk factor for BPH; individuals with increased WC and body fat ratio may have a higher risk of BPH.
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Objective To investigate the effects of weight-loss intervention on metabolic indicators in over-weight or obesity adults.MethodsA total of 116 over-weight or obese adults entered into this study from March 2008 to December 2009.The participants were divided into three groups by age.Each group received 6 months'weight-loss intervention.Body mass index(BMI),body fat rate,blood pressure,fasting blood glucose(FBG),oral glucose tolerance test(OGTY)2 h blood glucose,serum triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol (LDL-C)were measured before and after the intervention.The data were analyzed using t test.Results After the intervention,BMI,FBG,TG,and LDL-C were significantly decreased while HDL Was significantly increased in each group(P＜0.05).Body fat rate,systolic blood pressure,diastolic blood pressure and OGTT 2 h blood glucose were significantly reduced in the young and middle-aged groups(P＜0.05),however,there Was not significant difference in the older-age group.ConclusionsWeight-loss intervention may be effective in improving physical and metabolic indicators in adults with over-weight and obesity and reducing the risk of obesity-related diseases.